RSV-A (A2 Strain) Purified Virus
Catalog #: 10-247-100
Size: 100 µg
Biological Substance B
Human Respiratory Syncytial Virus subtype A (A2 Strain) propagated in HEp-2 cells and purified from culture supernatant and cells showing cytopathic effect.
≥ 5 x 109 viral particles per mL of purified RSV-A
For research use only.
APPLICATION FOR USE
- In vitro Infectivity Assay
- Antiviral Studies
- Animal Experimentation
- Nucleic Acid Extraction
- Spiking Studies
- ELISpot Assay
- Virus Neutralization Assay
- Virus Ultrastructural Studies
QUALITY CONTROL TESTING INCLUDES
- Sterility
- TCID50
- Protein Concentration
- Virus Particle Count
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
RECOMMENDATIONS:
Before use, rapidly thaw frozen vial in a water bath set at 37°C and then store on ice. Use pre-cooled medium/buffer for virus dilution and store on ice before inoculating into cell culture. For future use, aliquot into small amounts and freeze rapidly in a dry ice/alcohol bath and store at -70°C. Do NOT store virus stock at 2–8°C.
SAFE HANDLING RECOMMENDATIONS:
This preparation of RSV is a BIOHAZARDOUS material containing ACTIVE VIRUS and should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
REFERENCE ARTICLES
You, D., Siefker, D., Shrestha, B., Saravia, J., and Cormier, S. (2015). Building a better neonatal mouse model to understand infant respiratory syncytial virus disease. Respiratory Research 16:91. https://doi.org/10.1186/s12931-015-0244-0
Saravia, J., You, D., Shrestha, B., Jaligama, S., Siefker, D., Lee, G., Harding, J., Jones, T., Rovnaghi, C., and Bagga, B. et al. (2015). Respiratory Syncytial Virus Disease Is Mediated by Age-Variable IL-33. PLOS Pathogens 11, e1005217. https://doi.org/10.1371/journal.ppat.1005217
Cormier, S., Shrestha, B., Saravia, J., Lee, G., Shen, L., DeVincenzo, J., Kim, Y., and You, D. (2014). Limited Type I Interferons and Plasmacytoid Dendritic Cells during Neonatal Respiratory Syncytial Virus Infection Permit Immunopathogenesis upon Reinfection. Journal Of Virology 88, 9350-9360. https://doi.org/10.1128/jvi.00818-14
Warren, K., Simet, S., Pavlik, J., DeVasure, J., Sisson, J., Poole, J., and Wyatt, T. (2016). RSV-specific anti-viral immunity is disrupted by chronic ethanol consumption. Alcohol 55, 35-42. https://doi.org/10.1016/j.alcohol.2016.08.001
- New Product
- Available as Custom Product-Discontinued
- Limited Quantities Remaining-Discontinued
- Call For Availability
- Biohazard Category A
- Biohazard Category B
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