RSV-A (A2 Strain) Purified Virus
Catalog #: 10-247-100
Size: 100 µg
In Stock
Biological Substance B
Human Respiratory Syncytial Virus subtype A (A2 Strain) propagated in HEp-2 cells and purified from culture supernatant and cells showing cytopathic effect.
≥ 5 x 109 viral particles per mL of purified RSV-A
≥ 106.0 TCID50 per mL of purified RSV-A
Typical lot size 50-200 vials
For research use only.
APPLICATION FOR USE
- In vitro Infectivity Assay
- Antiviral Studies
- Animal Experimentation
- Nucleic Acid Extraction
- Spiking Studies
- ELISpot Assay
- Virus Neutralization Assay
- Virus Ultrastructural Studies
QUALITY CONTROL TESTING INCLUDES
- Sterility
- TCID50
- Protein Concentration
- Virus Particle Count
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
RECOMMENDATIONS:
Before use, rapidly thaw frozen vial in a water bath set at 37°C and then store on ice. Use pre-cooled medium/buffer for virus dilution and store on ice before inoculating into cell culture. For future use, aliquot into small amounts and freeze rapidly in a dry ice/alcohol bath and store at -70°C. Do NOT store virus stock at 2–8°C.
SAFE HANDLING RECOMMENDATIONS:
This preparation of RSV is a BIOHAZARDOUS material containing ACTIVE VIRUS and should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
REFERENCE ARTICLES
Warren, K., Simet, S., Pavlik, J., DeVasure, J., Sisson, J., Poole, J., and Wyatt, T. (2016). RSV-specific anti-viral immunity is disrupted by chronic ethanol consumption. Alcohol 55, 35-42. https://doi.org/10.1016/j.alcohol.2016.08.001
Saravia, J., You, D., Shrestha, B., Jaligama, S., Siefker, D., Lee, G., Harding, J., Jones, T., Rovnaghi, C., and Bagga, B. et al. (2015). Respiratory Syncytial Virus Disease Is Mediated by Age-Variable IL-33. PLOS Pathogens 11, e1005217. https://doi.org/10.1371/journal.ppat.1005217
You, D., Siefker, D., Shrestha, B., Saravia, J., and Cormier, S. (2015). Building a better neonatal mouse model to understand infant respiratory syncytial virus disease. Respiratory Research 16:91. https://doi.org/10.1186/s12931-015-0244-0
Cormier, S., Shrestha, B., Saravia, J., Lee, G., Shen, L., DeVincenzo, J., Kim, Y., and You, D. (2014). Limited Type I Interferons and Plasmacytoid Dendritic Cells during Neonatal Respiratory Syncytial Virus Infection Permit Immunopathogenesis upon Reinfection. Journal Of Virology 88, 9350-9360. https://doi.org/10.1128/jvi.00818-14
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