KSHV/HHV-8 IgG Antibody ELISA Kit
Catalog #: 15-501-000
Size: 1 Kit
Price: $1,320
Anti-Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Immunoglobulin G Enzyme-Linked Immunosorbent Assay Kit.
Kit Components:
- Antigen-Coated 96-well ELISA plate
- 4X Specimen Diluent
- Positive Control Plasma
- Negative Control Plasma
- 20X Wash Buffer
- HRP Conjugate
- Conjugate Substrate
- Stop Solution
- ELISA Accessory Pack
- Package Insert (1 copy)
Advanced Biotechnologies uses our own purified HHV-8 virus to coat the ELISA Kit plates. Whole viral particles provide a complete collection of antigens, offering higher sensitivity for HHV-8 antibody detection. Our purified virus was prepared using our superior purification process to minimize non-viral factors and ensure high specificity.
For any product recommendations or safe handling recommendations, please refer to Kit Insert and Product Safety Data Sheet.
For research use only.
APPLICATION FOR USE
- Detection of KSHV/HHV-8 IgG Antibody
QUALITY CONTROL TESTING INCLUDES
- Kit Performance Test
SHIPPING AND STORAGE:
This product is shipped at 2-8°C. Store at 2-8°C upon receipt.
RECOMMENDATIONS:
SAFE HANDLING RECOMMENDATIONS:
This product should be handled in accordance with Good Laboratory Practices and any applicable guidelines. Refer to Kit Insert and Product Safety Data Sheet for safe handling recommendations.
How are your Test Kits supplied? All of our research test kits are supplied on cold packs. Kits include the necessary reagents and complete instructions for performing the assay and interpreting the results.
How should your Test Kits be stored? Store all kits at 2 - 8°C. Do not freeze.
Are quantity discounts and automatic regular shipments offered for your Test Kits? Many options are available. Contact us for more information.
What equipment and materials will I need to perform the assay? Following is a list of equipment and materials that are required and not supplied:
• Microplate reader capable of absorbance readings at 450 nm and 650 nm
• Pipettes and tips capable of delivering 2–20 μL and 200–1000 μL
• Multichannel pipette and tips capable of delivering 100–300 μL
• Dilution tubes for diluting samples and controls
• 96 well dilution tube rack
• Reagent reservoirs
• Microplate washing system (optional)
• 37°C incubator
• Distilled or deionized water
• 500 mL and 100 mL graduated cylinder or volumetric flask
• Timer
• Disinfectant: 0.5% sodium hypochlorite
How many samples can I test? One 96-well antigen-coated plate is supplied in each kit. The plate consists of 12 removable 8-well strips coated with whole viral extract. We recommend each run include duplicates of a Blank, Positive control, triplicates of a Negative Control, and test samples should be run in duplicates. If the entire plate is run at once, a total of 44 samples can be tested.
Can you detect antibodies to both latent and lytic phases of HHV-8? Our HHV-8 ELISA Kit utilizes purified whole HHV-8 virus extract as the coating antigen. Unlike other kits that use single viral protein as antigen, whole virus extract provides a complete collection of antigens, hence higher sensitivity (lower false negative) for HHV-8 antibody detection. Our kit will detect antibodies to both latent and lytic phases of HHV-8. In addition, our purified virus was prepared by Advanced Biotechnologies' superior purification process to minimize non-viral factors and provide high specificity.
REFERENCE ARTICLES
Lee, Y., Hung, P., and Lin, C. (2018). Seroepidemiology and phylogenetic analysis of human herpesvirus type 8 in injection drug users and men who have sex with men in northern Taiwan. Journal Of International Medical Research 030006051876474. https://doi.org/10.1177/0300060518764747
Logan, C., Todorof, K., Fiorillo, S., Campbell, T., Elder, J., Borok, M., Gudza, I., Gwanzura, L., Ndemera, B., and Lochhead, M. et al. (2016). Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi’s Sarcoma-Associated Herpesvirus. PLOS ONE 11, e0163616. https://doi.org/10.1371/journal.pone.0163616
- New Product
- Available as Custom Product-Discontinued
- Limited Quantities Remaining-Discontinued
- Call For Availability
- Biohazard Category A
- Biohazard Category B
