Human Immunodeficiency Virus type 1 (IIIB Strain) infected HuT 78 cells lysed with Triton X-100.
Typical lot size 25-100 vials
For research use only.
APPLICATION FOR USE
Other Protein-Based Assays
QUALITY CONTROL TESTING INCLUDES
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Before use, if any precipitate is observed, thaw the frozen vial in a waterbath set at 37°C, vortex to mix, centrifuge at 3000 RPM for 5 minutes at 4°C. Aspirate the supernatant carefully and use for the intended assay.
How are Virus Infected/Uninfected Cell Extracts supplied? All of our antigens are supplied frozen on dry ice.
How are Virus Infected/Uninfected Cell Extracts purified? Our purification methodology varies according to the individual product requirements, as do the buffers in which the antigens are frozen. We recommend that you contact us with your assay specifications so that we may suggest the appropriate form and purity.
How pure are the Virus Infected/Uninfected Cell Extracts? Advanced Biotechnologies offers a variety of antigens (in different purities) to address individual assay/cost requirements. We recommend that you contact us with your assay specifications so that we may suggest the appropriate form and purity.
How should Virus Infected/Uninfected Cell Extracts be stored? We recommend that all antigens be stored frozen at -70°C or below. Avoid multiple freeze-thaw cycles as this will affect antigen activity. Do not dilute and freeze — make final dilution just prior to use.
How is the protein concentration of ABI’s Virus Infected/Uninfected Cell Extracts determined? A modified BCA protein assay is used with Bovine Serum Albumin (BSA) as the protein standard.