EBV (B95-8 Strain) Quantitated Viral DNA
Catalog #: 08-926-000
Size: 250 µL
Epstein-Barr Virus type 1 (B95-8 Strain) Quantitated Viral DNA PCR control.
Researchers who have viewed this product have also considered EBV (B95-8 Strain) Viral qDNA, catalog #17-926-500.
≥ 2,500,000 copies of EBV DNA per tube
For research use only.
APPLICATION FOR USE
- PCR
- Nucleic Acid-Based Assay
- Quantitative PCR Standard
QUALITY CONTROL TESTING INCLUDES
- PCR
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
RECOMMENDATIONS:
Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid. CAUTION: ABI does not recommend storage of dilutions of quantitated PCR Controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves may tend to lose copy number with time (sometimes a matter of an hour or so after dilution), especially at dilutions less than 100-1000 copies per microliter.
SAFE HANDLING RECOMMENDATIONS:
The DNA extraction procedure used has been shown to eliminate the infectivity of most viruses and bacteria; therefore, this product is not considered biohazardous. However, this product is not specifically tested for infectivity and should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.
How are Quantitated PCR controls supplied? Quantitated PCR controls are supplied at 1-2.5 × 104 copies/µL (with the exception of BK and JC polyomaviruses and PCV2, which are supplied at 1-2.5 × 105 copies/µL). The copy number of Quantitated PCR controls is determined by real-time PCR.
How can dilutions of Quantitated PCR controls be stored? ABI does not recommend storing dilutions of these controls under any conditions. All dilutions should be made immediately before use and used promptly. We have observed that dilutions used for standard curves typically lose copy number with time — sometimes as soon as an hour or so after dilution. This is especially common at dilutions less than 100-1000 copies per microliter.
REFERENCE ARTICLES
Agostini, S., Mancuso, R., Guerini, F., D’Alfonso, S., Agliardi, C., Hernis, A., Zanzottera, M., Barizzone, N., Leone, M., and Caputo, D. et al. (2018). HLA alleles modulate EBV viral load in multiple sclerosis. Journal Of Translational Medicine 16:80. https://doi.org/10.1186/s12967-018-1450-6
Nikitskaya, E., Lebedeva, A., Ivanova, O., Maryukhnich, E., Shpektor, A., Grivel, J., Margolis, L., and Vasilieva, E. (2016). Cytomegalovirus?Productive Infection Is Associated With Acute Coronary Syndrome. Journal Of The American Heart Association 5, e003759. https://doi.org/10.1161/jaha.116.003759
Salloum, N., Hussein, H., Jammaz, R., Jiche, S., Uthman, I., Abdelnoor, A., and Rahal, E. (2018). Epstein-Barr virus DNA modulates regulatory T-cell programming in addition to enhancing interleukin-17A production via Toll-like receptor 9. PLOS ONE 13, e0200546. https://doi.org/10.1371/journal.pone.0200546
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- Biohazard Category A
- Biohazard Category B
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