HIV-1 (IIIB Strain) gp160 Purified Native Glycoprotein
Oligomeric, fully-glycosylated, soluble native gp160 glycoprotein of Human Immunodeficiency Virus type 1 (IIIB Strain). The protein is probably truncated near the transmembrane domain and runs as a 140kD protein in SDS-PAGE. This viral protein was isolated from culture medium of HIV-1 infected H9 cells, lysed with 0.5% Triton X-100, and purified by immunoaffinity chromatography using mouse monoclonal antibody to HIV-1 gp41.
For research use only.
APPLICATION FOR USE
- Western Blot
- Dot Blot
- Other Protein-Based Assays
- Antibody Screening/Titration
- Antibody Production
QUALITY CONTROL TESTING INCLUDES
- Western Blot
- Protein Concentration
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
SAFE HANDLING RECOMMENDATIONS:
This inactivated biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
All proteins are supplied frozen on dry ice.
Our purification methodology varies according to the individual product requirements, as do the buffers in which the proteins are frozen.
We recommend that all proteins be aliquoted into single-use size portions and stored frozen at -70°C or below. Avoid multiple freeze-thaw cycles as this will affect protein activity. Do not dilute and freeze – make final dilution just prior to use.
Native proteins are made in infected cells using cell culture. Recombinant proteins are made in various systems, generally yeast or baculovirus. All are screened for reactivity in ELISA and Western blot prior to selection.
All of our proteins work in ELISA. However, it is critical to use an appropriate primary antibody and conjugate dilution. Double-block titrations should be performed to determine the optimum use dilutions in your system.
All of our proteins work in Western blot according to the considerations mentioned for ELISA.
It is not uncommon that different molecular weights are detected by electrophoresis due to different conformations. Please refer to the certificate of Analysis of each protein for its reported molecular weight.
A modified BCA protein assay is used with Bovine Serum Albumin (BSA) as the protein standard.