Custom Virus Inactivation
Since inactivated viral and bacterial preparations are essential for some investigations, ABI offers custom inactivation for any infectious agents in our inventory. Additionally, inactivations can be performed on samples submitted to ABI, or on custom viral or bacterial preparations.
ABI specializes in the following inactivation methods:
- Beta-Propiolactone (ß-PL): A chemical inactivation method that modifies the nucleic acid and leaves intact virus particles. ß-PL Inactivation causes a minimal or no modification of viral proteins.
- Detergent: A chemical inactivation method that leaves no intact virus particles. The detergent solubilizes all virus proteins.
- Heat: A physical inactivation method that leaves intact virus, bacterial, or Chlamydia EB particles. The heat inactivates the infectious agents, but leaves the nucleic acid intact.
- Ultraviolet (UV): A physical inactivation method that leaves intact virus particles. UV light inactivates the product's nucleic acid with minimal or no modification of the viral proteins.
As with all of our other preparations, Quality Control (QC) testing is a critical part of ABI’s production process. Validation of inactivation, Sterility, and Mycoplasma testing is performed on every custom inactivation product. In addition, the following QC assays are available:
- ELISA
- Protein Concentration
- SDS- PAGE
- Virus Particle Count by Transmission Electron Microscopy (TEM)
- Western Blot
- PCR
Other assays may be available on request.
Contact ABI for information on recommended inactivation methods and QC assays, order minimums, pricing, and scheduling.
Production Times and Order Minimums
Because each custom product is unique, production times and minimum quantities for product inactivation vary according to custom specifications. The below minimums are typical for custom inactivation orders; contact ABI for specifics regarding your project.
Production Time
- Please allow a minimum of 4-6 weeks for each custom inactivation.
Order Minimum
- For custom inactivation of standard products: 5 mg
- For custom inactivation of custom production products: 10 mg
