DNA Controls for PCR

ABI’s DNA controls can be used as positive controls in nucleic acid amplification reactions. Quantitated controls can also be used to generate standard curves for quantitative PCR assays.

ABI supplies virus infected cell DNAs, virus transformed cell DNAs, and quantitated DNAs.  Non-quantitated DNAs are purified from appropriate starting material, and then the concentration is determined by fluorometry using PicoGreen® dye.  Quantitated DNAs are prepared from purified viral or other organism DNA, then the copy number is determined by real time PCR using the Roche LightCycler®.  Custom Nucleic Acid Preparations are also available.

ABI’s DNA controls are purified from one of six different sources

  • Viral Particles
  • Infected Cells: transduced or pathogen infected cell cultures
  • Cultured Bacteria: pelleted bacteria from liquid, plate, or slant culture
  • Transformed Cells: cells transformed by specific viruses
  • Cultured Cells: cells used to propagate virus
  • Cloned Virus

Virus infected cell DNAs are isolated from virus infected cells and contain cellular and viral DNA in unknown proportions. Virus transformed cell DNAs are isolated from cells transformed by human papillomavirus (HPV) type 16 or type 18 and contain HPV viral DNA integrated into the cell genome.  Both virus infected and virus transformed DNAs are supplied at a DNA concentration (viral plus cellular DNA) of 50 ng/µL.

Quantitated DNAs for viruses, bacteria, and other organisms such as Toxoplasma gondii and Mycoplasma pneumoniae are also available.  Quantitated DNAs are supplied in volumes of 250 µL at 1-2 x 104 DNA copies/µL (except for BK and JC polyomaviruses, which are supplied at 1-2 x 105 DNA copies/µL).

Note: Quantitation with different instruments and by different methods may not give the same copy number results.  ABI’s DNA Controls are for research use only and are not for use in diagnostic assays.

Catalog # Product Namesort icon Size Storage Bio Cat. Status
08-730-100 Adenovirus-2 Infected Cell DNA 100 µL -20˚C
08-947-250 Adenovirus-5 Quantitated Viral DNA 250 µL -20˚C
08-942-250 BKV MM Strain Quantitated Viral DNA 250 µL -20˚C
08-931-000 Chlamydia trachomatis LGV-II 434 Strain Quantitated DNA 250 µL -20˚C
08-932-250 Chlamydophila pneumoniae CDC-CWL-011 Strain Quantitated DNA 250 µL -20˚C
08-925-000 CMV AD169 Strain Quantitated Viral DNA 250 µL -20˚C
08-926-000 EBV B95-8 Strain (Type 1) Quantitated Viral DNA 250 µL -20˚C
08-945-250 HHV-6A GS Strain Quantitated Viral DNA 250 µL -20˚C
08-923-000 HHV-6B Z-29 Strain Quantitated Viral DNA 250 µL -20˚C
08-950-250 HHV-7 H7-4 Strain Quantitated DNA Control 250 µL -20˚C New ProductNew product
08-707-000 HIV-1 IIIB Strain Infected Cell DNA 100 µL -20˚C
08-708-000 HIV-2 NIH-Z Strain Infected Cell DNA 100 µL -20˚C
08-754-100 HPV-16 Transformed Cell DNA 100 µL -20˚C
08-755-100 HPV-18 Transformed Cell DNA 100 µL -20˚C Call for availabilityCall for availability
08-921-000 HSV-1 MacIntyre Strain Quantitated Viral DNA 250 µL -20˚C
08-922-000 HSV-2 G Strain Quantitated Viral DNA 250 µL -20˚C
08-711-000 HTLV-I MT-2 Strain Infected Cell DNA 100 µL -20˚C
08-710-000 HTLV-II C3-44/Mo Strain Infected Cell DNA 100 µL -20˚C
08-943-250 JCV MAD1 Strain Quantitated Viral DNA 250 µL -20˚C
08-938-250 KSHV/HHV-8 KS-1 Strain Quantitated Viral DNA 250 µL -20˚C
08-949-250 Mycobacterium tuberculosis Quantitated Bacterial DNA 250 µL -20˚C New ProductNew product
08-946-250 Mycoplasma pneumoniae Quantitated DNA 250 µL -20˚C
08-924-000 Neisseria gonorrhoeae Quantitated Bacterial DNA 250 µL -20˚C
08-948-250 Toxoplasma gondii RH Strain Quantitated DNA 250 µL -20˚C
08-940-250 Vaccinia Lister Quantitated Viral DNA 250 µL -20˚C
08-927-000 VZV Rod Strain Quantitated Viral DNA 250 µL -20˚C
CSV