HIV-1 IIIB Strain gp160 env Purified Native Glycoprotein

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Catalog Number: 
14-103-050
Status: 
Current product--usually ships within 2 business days
Size: 
50 µg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C

Product Description: Soluble native gp160 from HIV-1IIIB infected H9 cells. This oligomeric, fully glycosylated native viral protein is isolated from conditioned cell culture medium inactivated with 0.5% Triton X-100 and purified by immunoaffinity chromatography using a mouse monoclonal antibody to HIV-1 gp41.

Protein Concentration: [varies by lot] µg/ml determined by BCA protein assay method using BSA standard. Alternative methods for determining protein concentration may give different results.

Final Fill Volume: [varies by lot]

Presentation: Purified native glycoprotein, gp160 of HIV-1IIIB, is supplied in PBS and is typically oligomeric.

Purity: 95% pure as estimated by analysis of Coomassie blue stained SDS- PAGE.

Functional Activity: Immunoreactive in ELISA1 and Western blot using HIV-1 antibody positive human sera. For ELISA, 10-100 ng protein and for Western bot, 100ng - 2 ?g protein is recommended depending on the nature and affinity of the detection reagent.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This product has been produced and purified in the absence of infectious virus preparation. However, the protein is biologically active and could potentially generate a protein-specific immune response if an exposure incident were to occur. Therefore, ABI recommends handling this material under BSL-2 guidelines set forth in NIH-CDC HHS publication number (CDC) 93-8395.