HSB-2 Uninfected Cell Extract

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Catalog Number: 
10-529-001
Status: 
Current product--usually ships within 2 business days
Discount: 
Quantity discounts are available for quantities of 5 or more. Please contact us for details.
Size: 
1.0 mg
Use: 
For research use only. Not for use in diagnostic procedures.
Storage Temperature: 
-70˚C
Quality Control Testing Includes: 
Yes Sterility
Yes ELISA
Yes Western blot
Yes Protein Concentration

HSB-2, a human T-lymphoblastoid cell line, is susceptible to infection with human herpes virus 6, variant A (HHV-6A) GS strain.

Cell Line for Propagation: HSB-2

Suspending Buffer: 100 mM Glycine, pH 9.5 with 0.05% Triton X-100

Final Fill Volume: Varies by lot

Protein Concentration: 1.2 mg/mL determined by Pierce BCA protein assay using BSA standard. Alternative methods for determining protein concentration may give different values.

Sterility: Bacteria, yeast, and fungi were not detected by our assay methods.

ELISA Testing: [varies by lot] units/mL — HHV-6A, strain GS Viral Lysate [varies by lot] units/mL — Uninfected HSB-2 Cell Extract. A unit is defined as the reciprocal dilution that gives an absorbance of 0.500 in our test system.

Recommended Use: This preparation of uninfected HSB-2 cell extract may be used as a negative control antigen in Immunoassays using viral antigen prepared from HSB-2 cells.

Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
Advanced Biotechnologies Inc recommends that all uninfected (human and mammalian) normal and human tumor cell lines be handled under Biosafety Level 2 (BSL-2) guidelines as defined in HHS publication No. 93-8395.