HSV-1 MacIntyre Strain Inactivated Cell Extract
Sterility
Mycoplasma
TCID50
ELISA: 20,000-25,000
Protein Concentration Cell Line for Propagation: Vero
Presentation: 100 mM Glycine, pH 9.5, 0.5% Triton X-100 100 mM Glycine, pH 9.5, 0.5% Triton X-100
Protein Concentration: [varies by lot] mg/mL by Pierce BCA protein assay method using BSA standard. Alternative methods for determining protein concentration may give different values
Validation of Inactivation: This lot of inactivated cell extract has been specifically tested for residual infectivity and no infectious virus was detected. The extract was titered in Vero cells over a 7-day period with endpoint determination by cytopathic effects (CPE). This product was treated using methods of inactivation that have been validated in vitro for complete inactivation of high-titered herpesviruses.
Recommendation: Before use, thaw the frozen vial in a waterbath set at 37°C, vortex to mix, centrifuge at 3000 RPM for 5 minutes at 4°C. Aspirate the supernatant carefully and use for the intended assay.



