Rat MAb to KSHV/HHV-8 ORF 73 (LNA-1)
Rat monoclonal antibody to latent nuclear antigen (LNA-1) encoded by ORF 73 of Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8. This antibody originates from ascites fluid and is purified by protein G chromatography.
Product to be discontinued. Last shipping day 6/29/2018.
For research use only.
APPLICATION FOR USE
- Dot blot
- Other Protein-Based Assays
- Flow Cytometry
- Antigen Detection
QUALITY CONTROL TESTING INCLUDES
- Protein Concentration
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
SAFE HANDLING RECOMMENDATIONS:
This biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
Garrigues, H., Howard, K., Barcy, S., Ikoma, M., Moses, A., Deutsch, G., Wu, D., Ueda, K., and Rose, T. (2017). Full-Length Isoforms of Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Accumulate in the Cytoplasm of Cells Undergoing the Lytic Cycle of Replication. Journal Of Virology 91, e01532-17. https://doi.org/10.1128/jvi.01532-17
Gupta, A., Oldenburg, D., Salinas, E., White, D., and Forrest, J. (2017). Murine Gammaherpesvirus 68 Expressing Kaposi Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen (LANA) Reveals both Functional Conservation and Divergence in LANA Homologs. Journal Of Virology 91, e00992-17. https://doi.org/10.1128/jvi.00992-17
Bruce, A., Barcy, S., DiMaio, T., Gan, E., Garrigues, H., Lagunoff, M., and Rose, T. (2017). Quantitative Analysis of the KSHV Transcriptome Following Primary Infection of Blood and Lymphatic Endothelial Cells. Pathogens 6, 11. https://doi.org/10.3390/pathogens6010011
Williamson, S., Nicol, S., Stürzl, M., Sabbah, S., and Hislop, A. (2016). Azidothymidine Sensitizes Primary Effusion Lymphoma Cells to Kaposi Sarcoma-Associated Herpesvirus-Specific CD4+ T Cell Control and Inhibits vIRF3 Function. PLOS Pathogens 12, e1006042. https://doi.org/10.1371/journal.ppat.1006042
Li, W., Yan, Q., Ding, X., Shen, C., Hu, M., Zhu, Y., Qin, D., Lu, H., Krueger, B., and Renne, R. et al. (2016). The SH3BGR/STAT3 Pathway Regulates Cell Migration and Angiogenesis Induced by a Gammaherpesvirus MicroRNA. PLOS Pathogens 12, e1005605. https://doi.org/10.1371/journal.ppat.1005605
Toth, Z., Papp, B., Brulois, K., Choi, Y., Gao, S., and Jung, J. (2016). LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection. PLOS Pathogens 12, e1005878. https://doi.org/10.1371/journal.ppat.1005878
Togi, S., Nakasuji, M., Muromoto, R., Ikeda, O., Okabe, K., Kitai, Y., Kon, S., Oritani, K., and Matsuda, T. (2015). Kaposi's sarcoma-associated herpesvirus-encoded LANA associates with glucocorticoid receptor and enhances its transcriptional activities. Biochemical And Biophysical Research Communications 463, 395-400. https://doi.org/10.1016/j.bbrc.2015.05.080
Chen, W., and Dittmer, D. (2011). Ribosomal Protein S6 Interacts with the Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus. Journal Of Virology 85, 9495-9505. https://doi.org/10.1128/jvi.02620-10
Chen, W., Hilton, I., Staudt, M., Burd, C., and Dittmer, D. (2010). Distinct p53, p53:LANA, and LANA Complexes in Kaposi's Sarcoma-Associated Herpesvirus Lymphomas. Journal Of Virology 84, 3898-3908. https://doi.org/10.1128/jvi.01321-09
Rappocciolo, G., Hensler, H., Jais, M., Reinhart, T., Pegu, A., Jenkins, F., and Rinaldo, C. (2008). Human Herpesvirus 8 Infects and Replicates in Primary Cultures of Activated B Lymphocytes through DC-SIGN. Journal Of Virology 82, 4793-4806. https://doi.org/10.1128/jvi.01587-07
Lu, F., Day, L., Gao, S., and Lieberman, P. (2006). Acetylation of the Latency-Associated Nuclear Antigen Regulates Repression of Kaposi's Sarcoma-Associated Herpesvirus Lytic Transcription. Journal Of Virology 80, 5273-5282. https://doi.org/10.1128/jvi.02541-05
How are ABI’s Antibodies supplied? All ABI antibodies are supplied frozen.
How should I store ABI’s Antibodies? We recommend that all antibodies be aliquoted into single-use size portions and stored frozen at -20° C or below. Avoid multiple freeze-thaw cycles as this will affect antibody activity. Do not dilute and freeze – make final dilution just prior to use. When single-use aligquot volume is too small, glycerol can be added instead to make final 50% glycerol for long-term storage.
Will ABI’s Antibodies work in Western blot? Not all ABI antibodies work in Western blot. Please see the Certificate of Analysis for each antibody to determine its suitability for Western blot use.