Mouse MAb to KSHV/HHV-8 ORF59
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Mouse monoclonal antibody to lytic cycle-associated early protein (50 kDa), encoded by ORF 59 of Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8. It is identified as DNA polymerase processivity factor (nuclear PF-8 protein), which is expressed in PEL cell lines (BCBL-1, BCP-1, BC-3, KS-1) 6-48 hours post-TPA induction. This antibody originates from ascites fluid and is purified by protein G chromatography.
For research use only.
APPLICATION FOR USE
- Western blot
- Dot blot
- Other Protein-Based Assays
- Flow Cytometry
- Antigen Detection
QUALITY CONTROL TESTING INCLUDES
- Western blot
- Protein Concentration
SHIPPING AND STORAGE:
This product is shipped frozen on dry ice. Store at -20°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
SAFE HANDLING RECOMMENDATIONS:
This biological preparation should be handled in accordance with biosafety guidelines defined in the BMBL, NIH-CDC HHS Publication No. (CDC) 21-1112.
Garrigues, H., Howard, K., Barcy, S., Ikoma, M., Moses, A., Deutsch, G., Wu, D., Ueda, K., and Rose, T. (2017). Full-Length Isoforms of Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Accumulate in the Cytoplasm of Cells Undergoing the Lytic Cycle of Replication. Journal Of Virology 91, e01532-17. https://doi.org/10.1128/jvi.01532-17
Rappocciolo, G., Hensler, H., Jais, M., Reinhart, T., Pegu, A., Jenkins, F., and Rinaldo, C. (2008). Human Herpesvirus 8 Infects and Replicates in Primary Cultures of Activated B Lymphocytes through DC-SIGN. Journal Of Virology 82, 4793-4806. https://doi.org/10.1128/jvi.01587-07
How are ABI’s Antibodies supplied? All ABI antibodies are supplied frozen.
How should I store ABI’s Antibodies? We recommend that all antibodies be aliquoted into single-use size portions and stored frozen at -20° C or below. Avoid multiple freeze-thaw cycles as this will affect antibody activity. Do not dilute and freeze – make final dilution just prior to use. When single-use aligquot volume is too small, glycerol can be added instead to make final 50% glycerol for long-term storage.
Will ABI’s Antibodies work in Western blot? Not all ABI antibodies work in Western blot. Please see the Certificate of Analysis for each antibody to determine its suitability for Western blot use.