Who provides the primary and secondary antibodies for immunolabeling services? How much volume of antibody should I provide?
For immunolabeling experiments, regardless of what type of procedure is to be employed, it is the responsibility of the investigator to provide the primary antibody, as well as information regarding the recommended concentrations and/or dilutions to be used for the experiment.
ABI will provide the secondary antibody or conjugate used for immunolabeling the specimens.
We strongly recommend that the investigator provide at least 200 microliters of the antibody to be utilized for immunolabeling procedures, although the total volume needed does depend on both the type of service to be performed and the strength of the primary antibody being used.
Due to some of the complexities that are involved with successfully performing immunolabeling techniques, we ask that before sending in your samples for immunolabeling services, you call and discuss your project with us directly over the telephone.