10-270-000

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Name: 
RubellaHPV77 Purified Virus, UV-Inactivated
Storage Temperature: 
-70°C
Status: 
New Product
Virus: 
Rubella virus, strain HPV77
Cell Line for Propagation: 
Vero
Technical Data
Size: 
1 mg
Final Fill Volume: 
Varies by lot
Validation of Inactiviation: 
Confluent monolayers of Vero cells were infected with undiluted to 10-4 dilutions of inactivated virus stock in four replicates and incubated for a period of 7 days. Active Rubella virus stock of the same lot and mock-infected Vero cells were included as positive and negative controls, respectively, for the assay. Cultures were incubated for a period of 7 days, and examined microscopically for the evidence of virus-specific CPE. The culture supernatant of active and inactivated virus were tested by HA using day old chick RBC. Only the active virus culture supernatant was positive for HA.This lot of inactivated Rubella virus stock, undiluted to 10-4 dilutions, did not yield any infectious virus. The active virus stock of the same lot yielded infectious virus up to a 10-6 dilution.
Suspending Buffer: 
Phosphate Buffered Saline (PBS), 250 mM sucrose, 25 μg/ml gentamicin.
Protein Concentration: 
[varies by lot] mg/ml by Pierce BCA protein method using BSA standard. Alternative methods for determining protein concentration may give different values.
TCID_50 Titer: 
Varies by lot. contact ABI for details.
Virus Particle Count: 
Varies by lot. Contact ABI for details.
HA Titer: 
Varies by lot
Shipping and Storage: 
This product is shipped frozen on dry ice. Store at -70°C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.
*Note: Upon thawing, centrifuge the vial for a few seconds to remove residual droplets from the lid.
Safe Handling Recommendations: 
This inactivated biological preparation should be handled in accordance with Good Laboratory Practices and any applicable local guidelines.