Frequently Asked Questions - Immunoassay Stabilizer
The cost per test depends on the volume used to saturate the solid phase already coated with antigen or antibody. A sufficient volume of Immunoassay Stabilizer must be present to completely cover unbound places on protein coated solid phase. If, in an ELISA assay, 100 µL of protein is used to coat each well, then approximately 150 µL per well of Immunoassay Stabilizer should be used. If other coating volumes are used, adjust the volume of Immunoassay Stabilizer accordingly.
No. The reagent has been developed to use as provided and should not be diluted further. Diluting it will weaken the stabilization effects and will raise the background.
In theory, yes. However, each protein must be checked individually for its characteristics in the presence of Immunoassay Stabilizer.
Yes. Use Immunoassay Stabilizer according to the protocols found on the Immunoassay Stabilizer product page. Allow the membrane to drip dry at room temperature. Use it while it is still wet, or completely dry it for future use.
Protein in aqueous solution depends upon interactions with water molecules to hold its shape. Removing the water forces the protein to change its conformation state to attain a low molecular energy state. This causes previously functional epitopes to be less accessible. Immunoassay Stabilizer essentially replaces the water as the protein is dried, allowing it to retain much of its fully hydrated structure and function.
The benefits of using Immunoassay Stabilizer will vary by application, but can be expected to improve the performance of an assay and can reduce the cost of making the assay.
No. The Immunoassay Stabilizer contains a blocking protein.
Drying the protein is crucial to maintaining immunological activity over time. The optimum stability can be only achieved when well-dried protein is appropriately stored in airtight pouches along with desiccants/indicator.
No. Plastic zipper bags allow air and gas interchange, causing the protein to lose stability and activity. Heat-sealable, airtight pouches are recommended instead.
Yes. ABI can manufacture custom immunoassay stabilizer solutions with high quality ingredients of your choice to fit your assay requirements. A 100 L minimum applies to orders. Contact ABI for more information about ordering custom Immunoassay Stabilizer formulas.
