How do I know which type of immunolabeling procedure I need?

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The following is a general guideline for the type of immunolabeling procedure utilized, depending upon the location of the target protein.  Contact ABI to speak with a technical support representative about which immunolabeling procedure is most appropriate for your needs.

  • Pre-Embedding Immunolabeling: Pre-Embedding Immunolabeling is used primarily for the detection of cell-surface associated antigens, as well as external envelope antigens of budding or extracellular virus particles. The sample must be unfixed and shipped by overnight delivery service to ABI at 4°C.
  • Negative Stain Immunolabeling: Negative Stain Immunolabeling is performed on specimens found in concentrated suspensions, such as virus particles, cell fragments, macromolecules, and bacterial specimens. Both internal and surface-related target proteins on minute specimens can be immunolabeled using this procedure.
  • Post-Embedding Immunolabeling: Post-embedding labeling is used exclusively for the immunolabeling of intracellular proteins found within cells and tissues.